Low frequency of knockdown resistance mutation (L1014F) and the efficacy of PBO synergist in multiple insecticide-resistant populations of Anopheles gambiae in Ikorodu, Lagos State, Nigeria

Objective: We evaluated the susceptibility status of Anopheles gambiae in two communities Ikorodu, Lagos, Nigeria to DDT, deltamethrin, lambda cyhalothrin and bendiocarb.
 Methods: immature stages were collected from their habitats surveyed community allowed emerge before exposure adult females discriminating doses WHO insecticides including cyhalothrin, bendiocarb malathion. PBO synergistic bioassay was conducted for where mosquito samples showed resistance. PCR assay used detection kdr mutation mosquitoes.
 Results: Resistance DDT (40% 86%) (75% 84%) Oke-Ota Majidun respectively. Suspected resistance deltamethrin (94.9%) (93.5%) recorded mosquitoes susceptible malathion both communities. KDR (L1014F) locations though with a low frequency that significantly departs Hardy-Weinberg’s probability (P> 0.01). synergized able increase knockdown, percentage mortality restore full Conclusion: Results this study indicates metabolic mechanism is highly implicated different classes insecticide Ikorodu should be taken into consideration when implementing vector control activities area.
 Keywords: Malaria; gambiae; resistance; knockdown